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Direct effects of minoxidil on epidermal cells in culture.-- Some Hair Loss Blogs
J Invest Dermatol. 1984 Jan;82(1):90-3.
Direct effects of minoxidil on epidermal cells in culture.
Cohen RL, Alves ME, Weiss VC, West DP, Chambers DA.
Minoxidil induces generalized hair growth when administered systemically, or localized hair regrowth when applied topically to sites of hair loss in severe alopecia areata or male pattern hair loss. The pharmacologic mechanisms by which minoxidil stimulates hair growth are unknown. This study was designed to examine whether minoxidil has direct effects on neonatal murine epidermal cells in culture. In the presence of minoxidil, cultures showed a marked dose-dependent second peak of DNA synthesis 8-10 days after culture initiation. In addition, two morphologically distinct cell types appeared. Indirect immunofluorescence staining with keratin-specific antibody revealed cytoplasmic keratin fibers, suggesting the epidermal origin of these cells. Our experiments demonstrate that minoxidil can affect epidermal cells in culture by altering their growth pattern and phenotypic appearance.